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The analysis crew screened various drug libraries by organising a system during which they observed the daily transcription of the clock gene Bmal1 by means of monitoring of a bioluminescent rhythm using the firefly's bioluminescence (luciferase) gene. Each drug was added to a 384-properly plate containing human cells (U2OS) transfected with the luciferase gene. The proliferation of genetic knowledge from experiments has been pushed by the ever reducing cost of sequencing genetic information utilizing gene chip technologies. Though no dangerous uncomfortable side effects have been observed with BH4 therapy, Kass says, at present, the drug comes with a major value downside.But, he says, BH4 is understood to have some impact on this chemical pathway, so «there might be some combination from these two therapies,» which he also plans to study. 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